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Contents
연구성과
학술발표
본문
Effect of Biotic Elicitor Treatment on Growth Characteristics and Anti-oxidant Activities of Damnacanthus major Calli
- 작성자
- 관리자
- 작성일
- 2024-09-24 16:36:08
- 조회수
- 2496
- 첨부파일
○ 발표제목
Effect of Biotic Elicitor Treatment on Growth Characteristics and Anti-oxidant Activities of Damnacanthus major Calli
○ 발표자
Eun Bi Jang, So-yeon Oh, Miye Kwon, Ho Bong Hyun, Boram Go, Jong-Du Lee, Young-Min Ham, Weon-Jong Yoon, Ji-Gweon Park*
○ 학술대회명
2024 (사)한국식물생명공학회 정기학술발표회 및 총회
○ 발표일자 및 장소
2024.08.26. ~ 2024.08.28
○ 초록
Damnacanthus major is a shrub that grows naturally in Jeju. In our previous study, D. major callus treated with an abiotic elicitor(methyl jasmonate 100 µM) was used as a cosmetic ingredient because it had moisturizing effects. We treated bacteria, a type of biotic elicitor, to effectively improve the biomass of D. major callus. We evaluated growth characteristics, antioxidant and moisturizing effects according to the species and concentration of microorganisms and culture period. The biotic elicitor was treated with 2% (v/v) of JBRI-B23-045, 401, and 403 from Jeju Techno Park Biodiversity Research Institute. JBRI-B23-401 was also tested for culture period (2, 3 weeks) and concentration (0.2%, 2%). Fresh weight was highest in the untreated group, but all biotic elicior treated groups had higher fresh weight than the abiotic elicitor treated group. In the 2% treatment of 045 and 401, it was about 1.9 times higher than the abiotic elicitor treatment. Depending on the concentration of 401, fresh weight was 1.3 times higher in the 2% treatment group compared to the 0.2% treatment group. There was no significant difference in dry weight depending on the biotic elicitor species, also higher biomass was obtained than the abiotic elicitor treatment. When treated with 2% of 401, there was no significant difference in dry weight depending on the culture period. When treated with bacteria, the color of callus changed to dark yellow, the same as when treated with abiotic elicitor. Total poly-phenol contents(TPC) in the 401 treatment were 1.9 times higher (4.94 µg/ml) than in the untreated and abiotic elicitor treatments. When treated with 401, TPC increased with the culture period, showing the highest value (5.81 µg/ml) among the treatments when cultured for 3 weeks. ABTS scavenging showed low IC50 values (106.7 and 124.6 µg/ml, respectively) when treated with 2% 401 and 403, and the 3-week culture with 401 treatment had the lowest IC50 value (55.4 µg/ml). The results of this study provide a basis for further research to increase the biomass of D. major callus with enhanced anti-oxidant activities.
Effect of Biotic Elicitor Treatment on Growth Characteristics and Anti-oxidant Activities of Damnacanthus major Calli
○ 발표자
Eun Bi Jang, So-yeon Oh, Miye Kwon, Ho Bong Hyun, Boram Go, Jong-Du Lee, Young-Min Ham, Weon-Jong Yoon, Ji-Gweon Park*
○ 학술대회명
2024 (사)한국식물생명공학회 정기학술발표회 및 총회
○ 발표일자 및 장소
2024.08.26. ~ 2024.08.28
○ 초록
Damnacanthus major is a shrub that grows naturally in Jeju. In our previous study, D. major callus treated with an abiotic elicitor(methyl jasmonate 100 µM) was used as a cosmetic ingredient because it had moisturizing effects. We treated bacteria, a type of biotic elicitor, to effectively improve the biomass of D. major callus. We evaluated growth characteristics, antioxidant and moisturizing effects according to the species and concentration of microorganisms and culture period. The biotic elicitor was treated with 2% (v/v) of JBRI-B23-045, 401, and 403 from Jeju Techno Park Biodiversity Research Institute. JBRI-B23-401 was also tested for culture period (2, 3 weeks) and concentration (0.2%, 2%). Fresh weight was highest in the untreated group, but all biotic elicior treated groups had higher fresh weight than the abiotic elicitor treated group. In the 2% treatment of 045 and 401, it was about 1.9 times higher than the abiotic elicitor treatment. Depending on the concentration of 401, fresh weight was 1.3 times higher in the 2% treatment group compared to the 0.2% treatment group. There was no significant difference in dry weight depending on the biotic elicitor species, also higher biomass was obtained than the abiotic elicitor treatment. When treated with 2% of 401, there was no significant difference in dry weight depending on the culture period. When treated with bacteria, the color of callus changed to dark yellow, the same as when treated with abiotic elicitor. Total poly-phenol contents(TPC) in the 401 treatment were 1.9 times higher (4.94 µg/ml) than in the untreated and abiotic elicitor treatments. When treated with 401, TPC increased with the culture period, showing the highest value (5.81 µg/ml) among the treatments when cultured for 3 weeks. ABTS scavenging showed low IC50 values (106.7 and 124.6 µg/ml, respectively) when treated with 2% 401 and 403, and the 3-week culture with 401 treatment had the lowest IC50 value (55.4 µg/ml). The results of this study provide a basis for further research to increase the biomass of D. major callus with enhanced anti-oxidant activities.