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연구성과
학술발표
본문
Multiplication, elicitation and ROS scavenging activity of Tetragonia tetragonoides adventitious root cultures in air-lift bioreactor
- 작성자
- 관리자
- 작성일
- 2024-11-29 11:43:07
- 조회수
- 5749
○ 발표제목
Multiplication, elicitation and ROS scavenging activity of Tetragonia tetragonoides adventitious root cultures in air-lift bioreactor
○ 발표자
Jong-Du Lee#, Ho Bong Hyun#, Hwon Choi, Jong Hee Im, Sang-Chul Han, Yun-Beom Kim, Hyejin Hyeon, Eunbi Jang, Young-Min Ham, Ji-Gweon Park, and Weon-Jong Yoon*
○ 학술대회명
2024 한국식물생명공학회 정기학술발표회
○ 발표일자 및 장소
2024.08.26.(월)~2024.08.28.(수), 메종 글래드 제주
○ 초록
Tetragonia tetragonoides is a perennial herb, commonly known as New Zealand Spinach (NZS) belonging to the Tetragoniaceae, and grows wild along costal area of Jeju island. The roots and leaves of NZS have been used for gastrointestinal diseases, and it has recently studied the effect assessment for antioxidants, diabetes, and hypertension. In this study, we investigated the biomass and activity indicators of multiplication and Reactive Oxygen Species (ROS) scavenging from NZS adventitious root according to different concentration of methyl jasmonate (MJ), as an elicitor treatment (external stress factors). In case of the uniform multiplication factors for NZS adventitious root culture was when treated on MS salt, sucrose 30 g·L-1, and NAA 0.5 mg·L-1 for 8 weeks in an air-lift bioreactor vessel (stored in Jeju Endemic Plant Cell Line Bank, JBRI-S21-003). As collation of MJ treatment concentration, the growth pattern was characterized higher dry matter (6.32%) and growth index (23.32 times) when treated 50 μM MJ than control (non-treatment), 100 μM MJ, and 200 μM MJ. Total phenolics content, total flavonoid content, and ability to intracellular ROS scavenging activity measured using the dichloro-fluorescein diacetate (DCF-DA) assay in H2O2 stimulated HaCaT cells were supported their ability to mitigate oxidative stress. Based on the antioxidant assessment from optimal biomass accumulation of adventitious roots in this study could be ex situ conservation and cosmeceutical and pharmaceutical metabolites by using sustainable biosynthesis platform. Moreover, the attractive Jeju native plant cell line ingredients from the further studies could be one of ecofriendly high-quality products to maximize the pilot-scale in vitro controlled culture system.
Multiplication, elicitation and ROS scavenging activity of Tetragonia tetragonoides adventitious root cultures in air-lift bioreactor
○ 발표자
Jong-Du Lee#, Ho Bong Hyun#, Hwon Choi, Jong Hee Im, Sang-Chul Han, Yun-Beom Kim, Hyejin Hyeon, Eunbi Jang, Young-Min Ham, Ji-Gweon Park, and Weon-Jong Yoon*
○ 학술대회명
2024 한국식물생명공학회 정기학술발표회
○ 발표일자 및 장소
2024.08.26.(월)~2024.08.28.(수), 메종 글래드 제주
○ 초록
Tetragonia tetragonoides is a perennial herb, commonly known as New Zealand Spinach (NZS) belonging to the Tetragoniaceae, and grows wild along costal area of Jeju island. The roots and leaves of NZS have been used for gastrointestinal diseases, and it has recently studied the effect assessment for antioxidants, diabetes, and hypertension. In this study, we investigated the biomass and activity indicators of multiplication and Reactive Oxygen Species (ROS) scavenging from NZS adventitious root according to different concentration of methyl jasmonate (MJ), as an elicitor treatment (external stress factors). In case of the uniform multiplication factors for NZS adventitious root culture was when treated on MS salt, sucrose 30 g·L-1, and NAA 0.5 mg·L-1 for 8 weeks in an air-lift bioreactor vessel (stored in Jeju Endemic Plant Cell Line Bank, JBRI-S21-003). As collation of MJ treatment concentration, the growth pattern was characterized higher dry matter (6.32%) and growth index (23.32 times) when treated 50 μM MJ than control (non-treatment), 100 μM MJ, and 200 μM MJ. Total phenolics content, total flavonoid content, and ability to intracellular ROS scavenging activity measured using the dichloro-fluorescein diacetate (DCF-DA) assay in H2O2 stimulated HaCaT cells were supported their ability to mitigate oxidative stress. Based on the antioxidant assessment from optimal biomass accumulation of adventitious roots in this study could be ex situ conservation and cosmeceutical and pharmaceutical metabolites by using sustainable biosynthesis platform. Moreover, the attractive Jeju native plant cell line ingredients from the further studies could be one of ecofriendly high-quality products to maximize the pilot-scale in vitro controlled culture system.