○ 발표제목
Effects of Eurya emarginata Leaves on Anti-aging.

○ 발표자
So Yeon Oh†, Sung Chun Kim, Hyejin Hyeon, Ho Bong Hyun, Boram Go, Yong-Hwan Jung and Young-Min Ham††

○ 학술대회명
2022년 대한화장품학회 추계학술발표대회

○ 발표일자 및 장소
2022. 11. 25. The-K호텔 서울호텔

○ 초록
Eurya emarginata (E. emarginata) is an evergreen tree that grows in warm area and is distributed in the southern regions of Korea such as Jollanam-do, Gyeongsangnam-do, Ulleungdo, Jeju-do. It has been used as a medicine for diuresis and phlegm removal, and previous studies have reported anti-inflammatory and anticancer effects. However, studies on antioxidant and anti-photoaging have not yet been reported. In this study, we investigated the effects of the E. emarginata leaf extract on antioxidant and anti-photoaging. The result of comparing the effects on the MMP-1 production of hot water extract (ELHW) and 70% ethanol extract (ELEE) in UVA-irradiated HDFn cells, the ELHW showed better inhibition of MMP-1 production even at a lower concentration than ELEE. In subsequent experiment, we fractionated ELHW and obtained the five fractions; hexane (Hex), chloroform (CHCl3), ethyl acetate (EA), butanol (BuOH) and water (H2O). As a result of comparing the antioxidant and MMP-1 production inhibitory effects of ELHW and five fractions, in the EA fr., the TPC and TFC were more than twice that of the EE, and the DPPH and ABTS radical scavenging activities were the best. Also, the EA fr. inhibited MMP-1 production the most. Additionally, we isolated three compounds from the EA fr.; Quercitrin (1), Eutigoside B (2), and Eutigoside C (3). Among the three compounds, Quercitrin showed excellent antioxidant activity and inhibition of MMP-1 production. Therefore, we suggest potential for use as a natural anti-aging agent of ELHW.