○ 발표제목
Validation of Optimized HPLC Method for Determining Flavonoids in Jeju Native Citrus Fruits from Different Harvest Times
○ 발표자
Hyejin Hyeon, Ho Bong Hyun, Boram Go, Sung Chun Kim, Seon-A Yoon, Yong-Hwan Jung, Young-Min Ham
○ 학술대회명
2023년도 (사)한국응용생명화학회 국제학술대회 및 제112차 정기총회
○ 발표일자 및 장소
2023.06.18.~19. 제주국제건벤션센터(ICC JEJU)
○ 초록
Jeju native Citrus have been used as functional ingredients due to their large quantities of flavonoids, and exhibit various biological activities such as antioxidant, antitumor, anti-inflammatory, and anti-obesity activities. In this study, we developed efficient quantification methods for the analysis of ten flavonoids in five types of Jeju Citrus fruits harvested at six different time points using HPLC. The parameter affecting the performance of HPLC method, such as column temperature, flow rate, detection wavelength, mobile phase buffer, were optimized. The established analytical method attained good linearity (R2 ≥ 0.9997), precision (inter-day precision, RSD ≤ 0.318%; intra-day precision, RSD ≤ 0.034%), and accuracy (95.06-107.97%). Analysis of flavonoids in Jeju native Citrus species from different harvesting time revealed that the Citrus samples were mainly characterized by the species, showing higher contents of hesperidin and narirutin in Citrus benikoji Hort. ex Tanaka (Gamja), Citrus pseudogulgul Hort. ex Shirai (Sadugam), Citrus junos Sieb. ex Tanaka (Soyuja), and higher contents of naringin and neohesperidin in Citrus grandis Osbeck (Dangyuja) and Citrus aurantium L. (Jigak). PCA results represented that the harvesting period also influenced the changes in flavonoid contents within each Citrus species group. This study demonstrated that our analytical method is practical and reliable for the quantification of Citrus flavonoids. This method could be further utilized to evaluate the quality of various Jeju Citrus fruits.