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Contents
연구성과
학술발표
본문
Antioxidant activity assessment with optimized biomass and elicitor treatment from adventitious root of Hibiscus hamabo Siebold & Zucc.
- 작성자
- 관리자
- 작성일
- 2023-09-14 08:58:26.0
- 조회수
- 2417
○ 발표제목: Antioxidant activity assessment with optimized biomass and elicitor treatment from adventitious root of Hibiscus hamabo Siebold & Zucc.
○ 발표자: 이종두, 윤선아, 현혜진, 고보람, 김성천, 장은비, 윤원종, 정용환, 함영민
○ 학술대회명: The 15th International Association for Plant Biotechnology Congress (IAPB 2023)
○ 발표일자 및 장소: 2023.08.06. - 08.11. 대전컨벤션센터(DCC)
○ 초록
Hibiscus hamabo Siebold & Zucc. (Yellow hibiscus) is a deciduous shrub plant, belonging to the Malvaceae, and inhabits mainly in coastal areas of Jeju island and south Jeonnam. Yellow hibiscus was newly classified as an observed species by Ministry of Environment, Korea from 2023. In our previous study, we confirmed the physiologically optimal culture condition of the adventitious root with biomass in air-lift bioreactor vessel and stored in Jeju Endemic Plant Cell Line Bank (JBRI-S20-003). In this study, we investigated the optimized biomass production and the antioxidant activity of yellow hibiscus adventitious root according to different concentration of methyl jasmonate, as an elicitor treatment method. In case of the uniform biomass accumulation for adventitious root culture was when treated on half strength MS salts, sucrose 30 g·L-1, and IBA 2 mg·L-1, for 8 weeks in a bioreactor vessel. As comparative investigation with four different methyl jasmonate concentrations, the growth pattern was characterized dry weight (3.61 g·L-1,) and growth rate (24.25 times) when treated the 50 μM treatment (low concentration) versus the control (non-treatment). It also revealed that the 50 μM treatment showed the most effective DPPH and ABTS radical scavenging activities. These assessments could be useful for developing the high-quality ingredient of yellow hibiscus adventitious roots to mass-proliferate pilot-scale biomass and bioactive compounds accumulation such as phenolic acids and flavonoids. Therefore, the cosmetic products from natural adventitious root cultures will be one of the ecofriendly manufacture procedures under in vitro controlled culture system without natural habitat destruction.
This work was supported by Korea Institute for Advancement of Technology (KIAT) through 2022 Regional Cooperation Innovation Growth Project (R&D) funded Ministry of Trade, Industry and Energy (MOTIE, Korea) (Grant number: P0021579)
○ 발표자: 이종두, 윤선아, 현혜진, 고보람, 김성천, 장은비, 윤원종, 정용환, 함영민
○ 학술대회명: The 15th International Association for Plant Biotechnology Congress (IAPB 2023)
○ 발표일자 및 장소: 2023.08.06. - 08.11. 대전컨벤션센터(DCC)
○ 초록
Hibiscus hamabo Siebold & Zucc. (Yellow hibiscus) is a deciduous shrub plant, belonging to the Malvaceae, and inhabits mainly in coastal areas of Jeju island and south Jeonnam. Yellow hibiscus was newly classified as an observed species by Ministry of Environment, Korea from 2023. In our previous study, we confirmed the physiologically optimal culture condition of the adventitious root with biomass in air-lift bioreactor vessel and stored in Jeju Endemic Plant Cell Line Bank (JBRI-S20-003). In this study, we investigated the optimized biomass production and the antioxidant activity of yellow hibiscus adventitious root according to different concentration of methyl jasmonate, as an elicitor treatment method. In case of the uniform biomass accumulation for adventitious root culture was when treated on half strength MS salts, sucrose 30 g·L-1, and IBA 2 mg·L-1, for 8 weeks in a bioreactor vessel. As comparative investigation with four different methyl jasmonate concentrations, the growth pattern was characterized dry weight (3.61 g·L-1,) and growth rate (24.25 times) when treated the 50 μM treatment (low concentration) versus the control (non-treatment). It also revealed that the 50 μM treatment showed the most effective DPPH and ABTS radical scavenging activities. These assessments could be useful for developing the high-quality ingredient of yellow hibiscus adventitious roots to mass-proliferate pilot-scale biomass and bioactive compounds accumulation such as phenolic acids and flavonoids. Therefore, the cosmetic products from natural adventitious root cultures will be one of the ecofriendly manufacture procedures under in vitro controlled culture system without natural habitat destruction.
This work was supported by Korea Institute for Advancement of Technology (KIAT) through 2022 Regional Cooperation Innovation Growth Project (R&D) funded Ministry of Trade, Industry and Energy (MOTIE, Korea) (Grant number: P0021579)