○ 발표제목: Metabolomics Reveals Rubiadin Accumulation and the Effects
of Methyl Jasmonate Elicitation in Damnacanthus major Calli

○ 발표자: Hyejin Hyeon, Eun Bi Jang, Sung Chun Kim, Seon-A Yoon, Boram Go, Jong-Du Lee, Ho Bong Hyun, Young-Min Ham

○ 학술대회명: 2024 한국대사체학회 정기학술대회 및 제12차 정기총회

○ 발표일자 및 장소: 2024.04.03.~05. 대구 EXCO

○ 초록
Callus suspension techniques have been considered attractive for improving bioactive metabolite productivity; methyl jasmonate (MeJA) is a widely used elicitor for stimulating synthetic pathways. In this study, a multivariate analysis-based metabolomics approach was employed to investigate the primary and specialized metabolites in the leaves, unelicited calli, and 100 or 200 µM MeJA elicited calli of Damnacanthus major. Rubiadin, a powerful anthraquinone with various therapeutic properties, was only identified in D. major calli, accumulating in a MeJA elicitation concentration-dependent manner. Callus cultures also contained high levels of amino acids, sugars, and phenolic compounds, indicating energy metabolism and metabolic adaptation responses for proliferation and stabilization. Regarding MeJA application, elicited calli contained higher amounts of quinic acid, kaempferol, and glucose with lower amounts of sucrose and raffinose than those in the unelicited control, which were closely related to protective mechanisms against MeJA. Moreover, excessive elicitation increased the asparagine, fructose, and raffinose levels and decreased the glucose and sucrose levels, which was ascribed to increased activation of the aminoacyl-tRNA biosynthesis pathway and wider utilization of glucose than of fructose after sucrose degradation. These results will be useful for optimizing plant cell culture techniques to achieve high production rates for valuable specialized metabolites.